Octoman is a representative of Takora, a planet currently at odds with the Milky Way Space Federation. The confrontation between the Federation and Takora grows more long and draw out by the day. The Takoraians are surrounded by enemies and are in a very unstable economic state. Due to this, Octoman wants to win the Grand Prix so that he can pay the education costs of his children. He plans to contribute the rest of his earnings to the government of Takora to give it hope for the future.

Mr. EAD is an android created by the mysterious development group EAD. His objective in this Grand Prix is to complete the final testing phase for his IQ and AI programming. AFter his run on the last F-ZERO Grand Prix, he endured many trials-combat training, spy activities, college exams, and many others. If all of his capabilities function as calculated, there should be nothing that can keep him from winning the Grand Prix. His victory will signify the birth of a flawless android.

McCloud is the leader of the flight squad Galaxy Dog. He had his combat plane converted into an F-ZERO machine in order to compete in the Grand Prix. During peacetime there is very little work available, so McCloud has entered his second race in an attempt to take home the big prize money. He is motivated to become the new champion by thoughts of his wife and young son, both of whom need his support.

One of Billy's ancestors was a trained rocket pilot and the first monkey in space. Whether by genetic predisposition or not, Billy's ability as a pilot is truly exceptional, and he's the first monkey to enter the F-ZERO Grand Prix. He was raised in a special lab where he was educated in human literacy and communication skills. The lab concurrently developed an F-ZERO racer suitable for a monkey and bestowed upon him the honor of piloting it in the Grand Prix.

Kate is the magestar singer who was chosen to sing at the opening ceremonies of the past several F-ZERO Grand Prix races. Her fans are wild about her powerful dance moves and soulful vocals. Completely allured by the extreme speed of the race, Kate chose to pursue F-ZERO racing rather than continue furthering her musical career. Taking full advantage of her sense of rhythm and athleticism, she plans to run a rambunctious race.

Zoda is in a long dispute with the hero of justice, Super Arrow. He has also been marked by Captain Falcon, and once was almost captured for his bounty. Zoda's crazed tendancies come from a special device that pumps adrenaline and dopamine into his body. He enters the Grand Prix races to raise funding for his sinister plots, and during the latest race, he plans to take out anyone who dares interfere with his master plan.

Jack is a former pro-group member who set the entertainment industry on fire by pursuing his dream to become an F-ZERO pilot, and he now wins the hearts of many female fans on the F-ZERO scene. Any merchandise bearing Jack's face is an instant sellout. While most people speak only of his good looks and popularity, his racing skills are also worty of comment.

Utilizing advanced biotechnologies, Bio Rex was created from a fossilized dinosaur egg. Genetic design allowed for the development of higher brain functions equivalent to that of a human, but as a carnivorous predator, he is still as aggressive as Pico. He first entered the Grand Prix to prove his superiority over humans, but this time around he has entered to satisfy his ravenous appetite for mammoth ribs.